LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1862..1958) /label=attL2 /note="attL2 (c)" misc_signal complement(1973..1992) /note="T7 Promoter/priming site (c)" primer_bind 2000..2016 /note="M13 Reverse priming site" gene 2129..2938 /note="Kanamycin resistance gene" rep_origin 3059..3732 /note="pUC origin" vector join(1870..3735,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1855 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:10632 IMAGE:3955396" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..1855 /gene="CHST12" /gene_synonym=C4S-2 /gene_synonym=C4ST-2 /gene_synonym=C4ST2 /db_xref="GeneID:55501" /db_xref="HGNC:17423" /db_xref="MIM:610129" CDS 692..1855 /dnas_title="CHST12 protein" /gene="CHST12" /gene_synonym=C4S-2 /gene_synonym=C4ST-2 /gene_synonym=C4ST2 /codon_start=1 /product="CHST12 protein" /protein_id="AAH02918.1" /db_xref="GI:12804131" /db_xref="GeneID:55501" /db_xref="HGNC:17423" /db_xref="MIM:610129" /translation="MTKARLFRLWLVLGSVFMILLIIVYWDSAGAAHFYLHTSFSRPH TGPPLPTPGPDRDRELTADSDVDEFLDKFLSAGVKQSDLPRKETEQPPAPGSMEESVR GYDWSPRDARRSPDQGRQQAERRSVLRGFCANSSLAFPTKERAFDDIPNSELSHLIVD DRHGAIYCYVPKVACTNWKRVMIVLSGSLLHRGAPYRDPLRIPREHVHNASAHLTFNK FWRRYGKLSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLR LYANHTSLPASAREAFRAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQI DYDFVGKLETLDEDAAQLLQLLQVDRQLRFPPSYRNRTASSWEEDWFAKIPLAWRQQL YKLYEADFVLFGYPKPENLLRD" misc_feature 692..1855 /note="CHST12 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgacagcgca ggcgccgcgc acttctactt 721 gcacacgtcc ttctctaggc cgcacacggg gccgccgctg cccacgcccg ggccggacag 781 ggacagggag ctcacggccg actccgatgt cgacgagttt ctggacaagt ttctcagtgc 841 tggcgtgaag cagagcgacc ttcccagaaa ggagacggag cagccgcctg cgccggggag 901 catggaggag agcgtgagag gctacgactg gtccccgcgc gacgcccggc gcagcccaga 961 ccagggccgg cagcaggcgg agcggaggag cgtgctgcgg ggcttctgcg ccaactccag 1021 cctggccttc cccaccaagg agcgcgcatt cgacgacatc cccaactcgg agctgagcca 1081 cctgatcgtg gacgaccggc acggggccat ctactgctac gtgcccaagg tggcctgcac 1141 caactggaag cgcgtgatga tcgtgctgag cggaagcctg ctgcaccgcg gtgcgcccta 1201 ccgcgacccg ctgcgcatcc cgcgcgagca cgtgcacaac gccagcgcgc acctgacctt 1261 caacaagttc tggcgccgct acgggaagct ctcccgccac ctcatgaagg tcaagctcaa 1321 gaagtacacc aagttcctct tcgtgcgcga ccccttcgtg cgcctgatct ccgccttccg 1381 cagcaagttc gagctggaga acgaggagtt ctaccgcaag ttcgccgtgc ccatgctgcg 1441 gctgtacgcc aaccacacca gcctgcccgc ctcggcgcgc gaggccttcc gcgctggcct 1501 caaggtgtcc ttcgccaact tcatccagta cctgctggac ccgcacacgg agaagctggc 1561 gcccttcaac gagcactggc ggcaggtgta ccgcctctgc cacccgtgcc agatcgacta 1621 cgacttcgtg gggaagctgg agactctgga cgaggacgcc gcgcagctgc tgcagctact 1681 ccaggtggac cggcagctcc gcttcccccc gagctaccgg aacaggaccg ccagcagctg 1741 ggaggaggac tggttcgcca agatccccct ggcctggagg cagcagctgt ataaactcta 1801 cgaggccgac tttgttctct tcggctaccc caagcccgaa aacctcctcc gagacTAGGA 1861 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 1921 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 1981 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2041 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2101 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2161 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2221 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2281 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2341 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2401 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2461 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2521 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2581 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2641 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2701 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 2761 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 2821 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 2881 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 2941 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3001 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3061 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3121 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3181 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3241 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3301 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3361 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3421 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3481 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3541 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3601 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3661 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3721 TTTTGCTCAC ATGTT //